The children's home, inspired by the Beatles' famous Strawberry Fields Forever song, is set to open its doors as a tourist attraction and youth center.
Music icon John Lennon "Resorted" in the Gardens of the Strawberry Field House in Woolton, Liverpool, as a little boy.
The plant was known to the Beatles fans, after she was immortalized by the band in 1967.
The page of the Salvation Army will be opened on Saturday.
Each year, an estimated 60,000 tourists visit the Beaconsfield Road site, which was run by the charity from 1936 to 2005. Since then it is closed.
Lennon's sister Julia Baird, 72, said that as a child, the musician would climb over the wall to get to "his special place."
"As children, we all have something special somewhere," she said.
About the project, Ms. Baird said, "I think he loved it because he was not a mainstream himself and was very aware of it."
The center will host an exhibition on Lennon's early life and train young people with learning difficulties.
Anthony Cotterill of the Salvation Army said, "John Lennon took refuge here as a child, and that is exactly what we want to offer by opening the strawberry field gates forever."