Bhutanese twins Nima and Dawa has been successful in Melbourne.
A team of medical experts emerged from theatre on Friday afternoon after the six-hour procedure, proudly declaring that the 15-month-old girls, who had joined the torso and shared a liver, had been separated.
The sisters are in recovery and recovery.
Head of Pediatric Surgery Dr Joe Crameri, who led the operation, said there have been no surprises, despite fears the girls' bowel may have been shared.
"We were very fortunate in that there was not any significant bowel attachment, and it was not all that much," Dr Crameri told reporters.
"And really the main challenge today, as we've thought reconstructed the abdomen so that both areas were closed over."
Surgeons were therefore able to die for life in ICU.
"There will be challenges over the next 24 to 48 hours as with any surgery," Dr Crameri said.
"We feel quietly confident that we have a good result and that is what I have just told me.
"We have to do a lot of monitoring things for a while to ensure that we achieve our aim."
Bhumchu Zangmo in October and the Children First Foundation retreat in Kilmore.
Ms Zangmo said to be "very relieved" by the result of the surgery.
The operation was not ready.
$ 350,000 and the state government has offered to pay the bill.
Other funds raised go to the twins' rehabilitation and return home.