A hound died on December 8 after a car accident on the A14.
The Fitzwilliam Hunt was traveling with dogs near Thrapston when the accident took place, and the North Cambs Hunt Saboteurs were also present.
It is assumed that the dog has hit the road before the collision, and it is assumed that no one else was injured.
A spokeswoman for Fitzwilliam Hunt said, "The Fitzwilliam (Milton) Hunt can confirm that today a hunting dog has driven onto the A14 and was killed in a car collision.
"The dog was completely killed, the driver was not injured and we thank her for her compassion and understanding after this unfortunate accident."
The hunt says the police were informed and Cambridgeshire live has contacted the Cambridgeshire Constabulary for comment.
The Fitzwilliam Hunt and the North Cambs Hunt Sabs have often collided in the past.
The BBC reports that Judge Clive Freedman issued a restraining order in November to stop the enemy hunting down the land used by the Fitzwilliam Hunt.
And in August, Barry Watson, 48, of Glatton, Cambridgeshire, admittedly inflicted attacks and criminal damage after torchlighting a car with saboteurs.