Italian third division side Pro Piacenza were knocked out of the league after beating only seven players in a 20-0 defeat on Sunday.
All seven players facing Cuneo were teenagers. The 18-year-old captain Nicola Cirigliano took over the position of manager.
Before the game on Sunday, they had not made three consecutive games.
The club, in serious financial difficulties and in last place in Serie A of Group A, was unable to pay its players and employees.
An eighth player could not start the game on Sunday after forgetting his identity papers, and the team's physiotherapist came on as a substitute.
On Monday, the league leaders found that some of the players were unregistered and disqualified the team from the competition, overhauling Cuneo 3-0.
Lega Pro also imposed a fine of 20,000 euros (17,500 GBP) on Pro Piacenza. He criticized the club's "unacceptable behavior" and described the game as "farce" and "dangerous".
The Italian Football Federation (FIGC) stated that membership of a club had been revoked because of "serious violations of the rules of the Cuneo Pro Piacenza game and numerous other irregularities".