The Scottish government has requested £ 52 million from an emergency fund to prepare for a Brexit without a deal.
Prior to the withdrawal of Great Britain from the European Union on 31 October funds were provided from the EU Exit Operational Contingency Fund.
The UK Government has announced that it will be available where Scotland has disproportionate costs.
Finance Minister Derek Mackay reiterated the Scottish Government's opposition to any form of Brexit.
He also demanded that the associated additional costs be covered for the period after the end of next month.
Mr. Mackay said, "The UK Government now seems to be actively seeking a no-deal outcome that is totally unacceptable and must be avoided at all costs.
"We have requested £ 52 million from the UK Government Fund to help us prepare for a no-deal outcome.
"This is the minimum operational requirement, but the true cost of a no-deal break will massively outweigh it and further funding will be needed."
He also said that leaving the EU was not Scotland's decision and demanded that the British government bear the costs involved.
Mr. Mackay added, "The Scottish Government should not have to cut spending on public services to fund preparations for Brexit.
"As a responsible government, we are already taking steps to protect jobs and our economy from a" no-deal "breach, and we will be presenting these plans to the Parliament shortly, but we face additional and disproportionate costs to address the implications of to mitigate such result.
"We will continue to stand in the EU and resolutely oppose efforts to combat our will."
The application includes funding to support the impact of no-deal on rural communities, increased demand for activities of Marine Scotland and Police Scotland, additional communication to EU citizens in the country, and poverty alleviation measures.
A Scottish Conservative spokesman said: "In 2016, the British electorate voted to leave the EU.
"Only the Scottish Conservatives have been working to prevent no-deal by supporting a deal.
"The SNP received £ 92 million for our councils to prepare for Brexit.
"However, there is no evidence that the Scottish local authorities have received anything."
After Scotland's chief economist had forecast on Friday a potential investment loss of £ 2 billion due to Brexit.
Forecasts for the period until April 2020 in the Scottish Government's quarterly State of the Economy report suggest that GBP 500m of investment could be offset if uncertainty continues with the year-end increase in the numbers.
A spokeswoman for the British government said: "We have given the Scottish Government almost £ 140 million to prepare for the EU withdrawal.
"We will review the continued bid by the Scottish Government as part of the Operational Contingency Fund in the usual way."