A picture of the "electrifying" first encounter between Boris Johnson and Jennifer Arcuri was revealed when the American businesswoman said she never wanted to be the girl who "showed up, shook him and left".
Ms. Arcuri said this week that she has a "very close bond" with the PM, but denied that the £ 126,000 in public funds she was awarded had anything to do with her friendship.
The picture of their first meeting was taken at a meeting of venture capitalists in central London when Mr. Johnson was mayor in October 2011.
At the moment when American businesswoman Jennifer Arcuri (front-right) met Boris Johnson for the first time at a meeting of venture capitalists in central London in October 2011
The event was featured in Johnson's Diary as a five-minute speech at the Landmark Hotel in Marylebone, the Guardian reported.
Referring to the first meeting with Mr. Johnson, Ms. Arcuri told ITV's Good Morning Britain, "I turned around and there's this guy walking around the room with disheveled hair and his shirt and papers that looked like I had them out my pulled out preschool backpack.
"And he comes across the room and keeps talking. And suddenly he turns the whole room of grim, angry men into howling schoolgirls. "
She added, "It was just electrifying to see that kind of personality and change all the energy in the room. Then I thought: Who is this guy? I have to say hello.
"And I went straight over and said, Mr. Johnson, my name is Jennifer Arcuri. I would really love to speak at my venture capital event. & # 39;
Mr. Johnson had time until Monday to explain why he wanted to keep the details of his relationship with entrepreneur Ms. Arcuri secret.
Jennifer Arcuri and Boris Johnson (pictured in 2014). During his tenure as Mayor of London, the Prime Minister signed the former model and the businesswoman around £ 126,000
The Prime Minister presented a statement to the London Assembly this week about his relationship with the blond former model, 34.
But the former Mayor of London, 55, sparked anger by labeling his answers "private" and "not intended for publication." The assembly demanded to know the reasons.
Len Duvall of Labor, chairman of the Assembly's oversight committee, wrote to the Prime Minister that he had "failed to provide the requested information" and gave him the Monday deadline.
Mr. Johnson has denied any inappropriateness in his role, but has repeatedly failed to have sex with the businesswoman who told the Mail this week that Mr. Johnson was her Henry VIII – and she was his Anne Boleyn.
"Anne Boleyn literally drove Henry VIII crazy for six years because she would not give him her virtue," said Ms. Arcuri. "She has never given too much away from herself, because she knew that if she did, what would have happened to her sister, would happen to her."
The revelation comes when Mr Johnson's lawyers have tried not to support an official investigation into his ties with Ms. Arcuri – claiming that they have no right to question him (photo: Ms. Arcuri in Back Boris 2012 campaign).
Ms. Arcuri states that all scholarships granted to her company were "above average" (picture: Miss Arcuri in a photo shoot in July 2013).
The Californian added that "the last thing she wanted to be was the girl who showed up, scolded him and left," and insisted that she was the one who put the ax on her "unique connection."
Johnson spoke at four events from Innotech, Ms. Arcuri's startup, and both visited Kuala Lumpur, New York and Tel Aviv on three EU-funded trade missions.
The revelation comes when Mr Johnson's lawyers have tried to stop supporting an official investigation into his ties with Ms. Arcuri – claiming that they have no right to question him.
Atkins Thomson, the law office of the Prime Minister, wrote a letter to the chairman of the Greater London Authority (GLA) Supervisory Committee, Len Duvall, after the panel asked for "full" details of Mr. Johnson's relationship with the 34-year-old.
The letter adds: "It appears that the GLA Supervisory Board is exceeding its remit or exceeding the mark by requiring information from our client."
The GLA Committee wrote to Mr. Johnson on allegations that as the mayor of London, he had "failed to register a potential conflict of interest with regard to the allocation of public funds."
The Authority called for "details and a timeline of all contacts with Jennifer Arcuri during her tenure as mayor of London, including personal, social and professional".
It came when Miss Arcuri gave the post an exclusive interview in which she described herself as "Boris & # 39; Anne Boleyn" and described how the second wife of Henry VIII had refused to "give him her virtue"
Jennifer Arcuri meets Boris Johnson at the Excel World Islamic Economic Forum, London, October 2013
Mr. Johnson responded this week by sending material to the committee – but it was labeled "private and confidential" and "not for publication." Mr. Duvall from the GLA committee has already written to him and asked why he has marked the information as secret.
Mr. Johnson has consistently denied any inappropriateness – however, he has uniquely failed to deny an affair with Miss Arcuri.
Miss Arcuri also says any grants given to her company were "overboard".
The letter sent by Mr Johnson's lawyers this week and sent to the Huffington Post stated that their client had "the intention of fully cooperating with the Director-General of the Independent Police Department Office in his separate investigation into the prime minister's ties to Arcuri cooperate ".